Xf setup

Measure oxygen and proton flux of cells grown in XF cell culture plates. The analyzer is used in conjunction with XF sensor cartridges. - XF Controller - Operation of the Analyzer is done through a high-resolution color LCD touch screen with stand, that may be installed in front of or beside the XF Pro. The controller communicates with the analyzer using a single serial cable and a single USB A-B cable. Suitable locations for the XF Pro system XF Pro Analyzers are designed for laboratory use. The internal environment of the analyzer is controlled to a preset temperature by the user; therefore, laboratory room temperature must be maintained within the range listed in the specification table. Sample temperature control performance can be monitored using the status display on the right side of the analyzer or on the Wave application display. The XF Pro uses optical detection technology to measure extremely low levels of fluorescent emission from analyte sensors. While the analyzer has been designed to shield room light, excessive light (such as direct sunlight) should be avoided. WARNING The electrical connection at the back of the XF Pro is the primary disconnect for the instrument. The XF Pro should be positioned to allow accessibility to the power cord for easy disconnection. CAUTION Avoid drafty areas, as well as areas experiencing significant vibration (such as a centrifuge). 18 Seahorse XF Pro Analyzer Operating Manual Installation 2 Internal components of the XF Pro Analyzer Internal components of the XF Pro Analyzer Removing the side doors reveals the measurement chamber in which the assay is conducted. The electro-optics hardware is enclosed in a card cage in the rear chamber, and this is connected to the probe head through a set of fiber optic cable bundles. The base of the enclosure contains the primary controller board and heater assembly. (See Figure 1 and Figure 2.) - LCD message screen - Displays current instrument action and setpoint temperature, and sample temperature. - Cover latches - Pull on indented hand-holds molded into the side doors (not shown) to lift them up, exposing the internal components of the instrument. Magnets hold in place. - Probe head and injection manifold - The probe head consists of 96 "light guides" to carry the optical signals to and from the sensors. The injection manifold uses compressed air to inject compounds loaded into sensor cartridge ports into the assay wells. Figure 1 XF Pro front/side view. Base color may vary. Fiberoptics Injection manifold Thermal tray Manifold heater LCD screen Cover latches (not shown) Seahorse XF Pro Analyzer Operating Manual 19 2 Installation Setup and interconnects: cable installation - Barcode reader - Reads barcode on sensor cartridge and cell plate. Setup and interconnects: cable installation The XF Pro Analyzer is operated from a touch screen computer monitor mounted to a stand, referred to as a controller. One RS232 cable and one USB cable handle the communication of commands and data between the instrument and the controller. The controller may be connected to

News |Phase One firmware updates XF system, promises unlimited shutter actuationss News You Might Also Like… Phase One has introduced a new firmware update to its medium format XF Camera System that adds Electronic Shutter integration, among other new features.Available now and free for all Phase One XF camera system owners, the new firmware update includes complete Electronic Shutter integration on the IQ3 100-megapixel system.And Phase One has extended its warranty on the XF Camera System to include unlimited shutter actuations.What’s more, the new Phase One firmware update adds enhancements for studio and flash photography with the in-camera Profoto Air Remote enabling Profoto TTL metering.The update also adds a post-capture Flash Analysis Tool to give even more control over the lighting. Full functionality of the Profoto Air for up to six groups is now built directly into the XF Camera System.Phase One has also announced a new XF V-Grip for the system and added new customisation options such as Icon Control, Create, Load & Save System Setup and Advanced Tool Options for sequence tools.Click here to download the new Phase One XF firmware update.The new XF V-Grip price tag will be $1,490 and will be released in November 2016.READ MOREHands-on Fuji GFX 50S review Hands-on Olympus OM-D E-M1 Mark II review Cameras Firmware Update Medium Format Cameras Stay connected Sign-up to receive our newsletter and information about Camera Jabber Photographer of the Year --> --> --> Camera Jabber Jabber Digital Media Ltd. - 4 Beau Street, Bath, BA1 1QY. All rights reserved.

Contact, and additional resource information for the Agilent Seahorse XF Pro Analyzer. Seahorse XF Pro Analyzer Operating Manual 3 This page intentionally left blank. 4 Seahorse XF Pro Analyzer Operating Manual Contents About This Manual 3 1 Introduction General Information 8 Safety Considerations 9 Electrical hazards 9 Safety Labels 10 Instrument Overview and Intended Use 11 Technical Specifications 12 2 Installation Unpacking and Component Identification 14 Installation Procedure 18 Suitable locations for the XF Pro system 18 Internal components of the XF Pro Analyzer 19 Setup and interconnects: cable installation 20 3 Basic Operation Power and Warm Up 24 Power up 24 Launch Wave 24 Performing XF assays 25 XF Pro status indicator 25 Wave Controller widgets 25 XF Pro maintenance 26 XF Pro Assays at Non-37 C Temperatures 28 Operational and assay guidelines for non-37 C assays 28 Set alarm (temperature tolerance range) 30 4 Maintenance Cleaning and Routine Maintenance 32 Seahorse XF Pro Analyzer Operating Manual 5 Troubleshooting 33 Barcode errors 33 Contact Information 36 Worldwide technical support 36 Ordering 36 Additional Resources 37 6 Seahorse XF Pro Analyzer Operating Manual 1 Introduction General Information 8 Safety Considerations 9 Instrument Overview and Intended Use 11 Technical Specifications 12 This chapter provides an introduction to the Agilent Seahorse XF Pro Analyzer. Seahorse XF Pro Analyzer Operating Manual 7 1 Introduction General Information General Information This manual covers all models of Agilent Seahorse XF Pro Analyzer: Instrument type Part Number(s) Seahorse XF Pro S7850A (manufacturing part reference #S7850-64000) 8 Seahorse XF Pro Analyzer Operating Manual Introduction 1 Safety Considerations Safety Considerations The XF Pro has been carefully designed so that when used properly the instrument is accurate, fast, flexible, and safe. Information on safety practices is provided with the instrument and operation manuals. Before using the instrument or accessories, thoroughly read these safety practices. Electrical hazards Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Agilent-trained, -qualified, or authorized service engineers. Consult the manuals or product labels supplied with the XF Pro to determine which parts are operator-accessible. Instrument must always be turned off and disconnected from all power before removing any outer covers. Replace all covers prior to reconnecting power. Always use a three-wire outlet with ground connection which is adequately rated for the load. The installation must comply with local safety regulations. Always use the supplied power cords. Use of power cord with inadequate ratings can create a fire hazard or cause damage to the instrument. WARNING Observe all relevant safety practices at all times. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Unskilled, improper, or careless use of this instrument can create shock hazards, fire hazards, or other hazards which can cause death, serious injury to personnel, or severe damage to equipment and property. WARNING The XF Pro contains electrical circuits, devices, and components operating at dangerous voltages. Contact with

An external network, through the ports on the underside. See Unpacking and Component Identification on page 14 to identify each cord, and refer to Figures 3 and 4 to identify the connectors. 1 Connect power cords - One power cord is used to connect the instrument to a grounded AC (mains) outlet. A second power cord is used to connect the controller power supply module to the AC supply. The power supply module is Figure 2 XF Pro front view. Barcode reader 20 Seahorse XF Pro Analyzer Operating Manual Installation 2 Setup and interconnects: cable installation then connected to the socket at the bottom of the controller. (See Figure 3.) 2 Connect the data cables to the analyzer. One RS-232 cable connects the controller serial port to the analyzer socket labeled "COM". (See Figure 4.) 3 A second cable (USB) connects the analyzer socket labeled "USB" to the USB port on the controller. This port must be used for proper functioning of the instrument and barcode reader. (See Figure 3.) 4 Connect external network cables. The controller may be networked via the Ethernet port on the controller. 5 Connect the AC power cord to the AC input on the XFe rear panel and then switch the power switch to the on position. (See Figure 5.) Figure 3 Controller ports (underside). Figure 4 XF Pro rear panel - USB and serial ports. USB RS-232 Seahorse XF Pro Analyzer Operating Manual 21 2 Installation Setup and interconnects: cable installation 6 Securely attach the cover and close the tray door. When the cover is securely attached and tray door is closed, optical switches are engaged to monitor the system. An optical sensor is also used to determine the status of the door. The XF Pro has a heater that maintains a stable internal system temperature. Typically, the temperature will be maintained at 37 C, as monitored by temperature sensors and controllers embedded in the tray and above the tray. A thermal fuse will disable the sample tray heater should it reach an abnormally high temperature. The manifold heater will not be disabled if this situation occurs, and the user should power off the instrument and contact technical support. In this situation the user should not attempt to open the instrument covers. Figure 5 XF Pro rear panel - AC input and power switch. Power switch I: on, O: off AC input WARNING The door opens automatically when the tray is extended, allowing the operator to insert or remove the well plate/cartridge consumables. The operator must exercise caution during the loading of the well plate/cartridge to avoid the possibility of a pinch hazard. After the well plate/cartridge is securely on the tray, the operator's hand must be removed from the area of the tray before continuing the assay. After the command is given to continue the assay via the controller, the tray will move slowly back into the instrument and the door will close. CAUTION Safe operation of the instrument requires that the cover

These circuits, devices, and components can cause death, serious injury, or painful electric shock. WARNING Connection of the instrument to an incorrectly wired supply outlet, or lack of proper electrical grounding can create a fire hazard or a potentially serious shock hazard and could seriously damage the instrument and any attached ancillary equipment. Seahorse XF Pro Analyzer Operating Manual 9 1 Introduction Safety Labels Safety Labels The following table lists the common safety labels you might find on the Agilent Seahorse XF Pro Symbol Description Indicates pinch, crush, or cut hazard. 10 Seahorse XF Pro Analyzer Operating Manual Introduction 1 Instrument Overview and Intended Use Instrument Overview and Intended Use The Agilent Seahorse XF Pro analyzer measures the rate of change of dissolved oxygen and pH in the media immediately surrounding living cells cultured in a microplate. Changes in the extracellular media are caused by the consumption or production of analytes by the cells. Therefore, a sensitive measurement of the media flux can be used to determine rates of cellular metabolism with great sensitivity and in a totally noninvasive, label-free manner. A unique feature of the Seahorse XF technology is its ability to make accurate and repeatable measurements in as little as five minutes. The instrument, working with a sensor cartridge, isolates a few L of media above the cell monolayer. Cellular metabolism causes rapid, easily measured changes to the "microenvironment" in this small volume. An XF sensor cartridge is required to run an assay. The cartridge has 96 probes, and each probe has a single multifluor sensor spot that is sensitive to both oxygen and proton concentration. The system measures the concentration of each analyte over time and automatically calculates the oxygen consumption rate (OCR) and proton efflux rate (PER) - which is a quantitative measure of extracellular acidification rate (ECAR) - simultaneously in every well of the microplate. Typically, a measurement cycle is performed for 6 minutes. The media is gently mixed, the probe is positioned 200 m above the well bottom, and the analyte levels are measured. Baseline metabolic rates are typically measured three to four times and are reported in pmol/min for OCR, mpH/min for ECAR, and pmol/min for PER. Compound is added to the media and mixed, and then the post-treatment OCR and ECAR measurements are made and repeated. As cells shift metabolic pathways, the relationship between OCR and ECAR/PER changes. The XF Pro system, comprising a bench top analyzer and touch screen controller, is driven by Agilent Seahorse Wave Pro software. This software enables all aspects of Seahorse XF assays including assay setup, instrument control, and data analysis. Consumables are sold separately and include Agilent Seahorse XF FluxPaks (comprising sensor cartridges, cell plates, and calibrant) as well as a variety of assay kits, reagents, and media. XF sensor cartridges are specific for the instrument type and can be purchased exclusively from Agilent. Seahorse XF Pro Analyzer Operating Manual 11 1 Introduction Technical Specifications Technical Specifications Model Number S7850A Controller Dimensions: Width height depth 21"